Hi,
Which secretion signal(s) did you use? The choice of secretion signal can
make or break the entire process.
Have you checked total yield of your protein in the cytoplasm and in the
medium? Can you make assumptions regarding the bottleneck - is it protein
synthesis/folding or is it the secreti
Hello Chen,
You did not mention perhaps the most important element that can make or
break the secretion process - namely the secretion signal. Which signal(s)
have you already tried?
Have you attempted to quantify the protein made in the total IC? In other
terms, is the bottleneck in the protein
Hi,
We are trying to express a protein in insect cells as secreted. However, the
yield is VERY low using either pFastBAC or pAcGP67A. We have tried many
different constructs and this is the only one that at least expresses. I wonder
whether anyone knows how to improve. Many thanks.
Best,
Ch
