Hi, Which secretion signal(s) did you use? The choice of secretion signal can make or break the entire process.
Have you checked total yield of your protein in the cytoplasm and in the medium? Can you make assumptions regarding the bottleneck - is it protein synthesis/folding or is it the secretion process itself? More details would be great to have! Artem > Hi, > > We are trying to express a protein in insect cells as secreted. However, > the yield is VERY low using either pFastBAC or pAcGP67A. We have tried > many different constructs and this is the only one that at least > expresses. I wonder whether anyone knows how to improve. Many thanks. > > Best, > Chen > > >
