CP4BB@JISCMAIL.AC.UK] On Behalf Of
Chaudhary, Ritcha
Sent: Friday, September 16, 2011 6:05 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Insoluble ligand?
Hi All
I have a ligand that is dissolved in 100% DMSO. The ligand crashes out
even when diluted to 20% final DMSO concentration in the protein b
Keep in mind it may also be possible to soak in
ligand from the solid if the binding constant is tight enough. Le
Chatelier's principle will drag it from the undissolved solid into
the complex. This process will be improved if you can increase
solubility in soluti
Hi Ritcha,
Did you try Methanol or Ethanol to see if your ligand dissolve in these
solvents? You should try these solvents first before adding any
detergents.
Good luck.
Smita Mohanty
>>> "Chaudhary, Ritcha" 9/16/2011 11:04 AM
>>>
Hi All
I have a ligand that is dissolved in 100% DMSO. The l
Hi All
I have a ligand that is dissolved in 100% DMSO. The ligand crashes out even
when diluted to 20% final DMSO concentration in the protein buffer. I have
heard of people using detergents such as n octyl Beta D glucoside to keep the
ligand solubilized at lower DMSO concentrations. Can someon
Simon,
We routinely obtain structures from protein solutions with a big pellet of
ligand in the bottom of the tube. For co-crystallizations we add 1mM
compound to a 0.3mM solution of the protein and incubate overnight. Many of
the compounds are only soluble to 50micromolar, so we get a lot of
pre
Dear Simon,
DMSO concentrations lower than 5% usually do not alter crystallizability
of a protein. In case you want to avoid this solvent may I suggest you
trying out two methods that worked for me.
1. If you grow crystals in PEGs or similar molecules you might try to
solubilize the compound in a
> HTH
>
> Carsten
>
>
>
> > -Original Message-
> > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED]
> > Behalf Of Yue Li
> > Sent: Tuesday, December 11, 2007 11:55 AM
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: [ccp4bb] insoluble ligand
&
>
>
>
> > -Original Message-
> > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED]
> > Behalf Of Yue Li
> > Sent: Tuesday, December 11, 2007 11:55 AM
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: [ccp4bb] insoluble ligand
> >
> >
> &g
Li
> Sent: Tuesday, December 11, 2007 11:55 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] insoluble ligand
>
>
> Hi all,
>
> I have one ligand which is insoluble in water, and I would like to
> co-crystallize it with my protein. Is there any other method
> except f
Hi all,
I have one ligand which is insoluble in water, and I would like to
co-crystallize it with my protein. Is there any other method except for
dissolving it in DMSO ?
Thanks
Simon
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