Hi Ritcha, Did you try Methanol or Ethanol to see if your ligand dissolve in these solvents? You should try these solvents first before adding any detergents.
Good luck. Smita Mohanty >>> "Chaudhary, Ritcha" <chaudha...@missouri.edu> 9/16/2011 11:04 AM >>> Hi All I have a ligand that is dissolved in 100% DMSO. The ligand crashes out even when diluted to 20% final DMSO concentration in the protein buffer. I have heard of people using detergents such as n octyl Beta D glucoside to keep the ligand solubilized at lower DMSO concentrations. Can someone give me pointers in this direction? Any suggestions, references etc..... Thanks much Ritcha
