Hi,
We are using a modified version of ISFM with 10 g/L glucose, based on
a paper by Maiorella et al. from around 1988 or 1989. This is a
medium that is based on IPL-41, which we purchase as a powder, and
supplemented with ultrafiltered yeastolate and lipids. It is
completely protein-free. It i
onday, June 07, 2010 11:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] insect cell media
I mentioned to to Chris already, but we use nothing but HyClone
SFX-Insect powder. We make 20-30 L batches, sterilize with a large
peristaltic pump and a disposable Millipak filter from Millipore. We
never
I mentioned to to Chris already, but we use nothing but HyClone
SFX-Insect powder. We make 20-30 L batches, sterilize with a large
peristaltic pump and a disposable Millipak filter from Millipore. We
never have contamination problems that are due to preparing our own
media from powder. We buy 10
Hi, well, actually this is availalbe as powder (HYClone), as it says
on the page.. (we have it made in our media kitchen on a regular
basis, but
not for huge scale... so i dont know about the prices..)
https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=LA11074__10347&
We have happily made a transition last year from using Invitrogen's
SFM medium and cellfectin to Insect-Xpress (Lonza) and
polyethyleneimine for transfection. We are moving several protein
targets to large-scale cultures and would consider cost-cutting
alternatives. For example, Invitrogen and The
Hi,
I would like to get some feedback on the type of media that people are
using for insect cell culturing.
We have happily made a transition last year from using Invitrogen's
SFM medium and cellfectin to Insect-Xpress (Lonza) and
polyethyleneimine for transfection. We are moving several pro
