Hi, We are using a modified version of ISFM with 10 g/L glucose, based on a paper by Maiorella et al. from around 1988 or 1989. This is a medium that is based on IPL-41, which we purchase as a powder, and supplemented with ultrafiltered yeastolate and lipids. It is completely protein-free. It is exceptionally cheap but does require a little more work than commercial media to prepare (you have to emulsify the lipids yourself).
The medium costs us $3.92 per L before filter sterilization, and $5.26 per L after. We use 2 Stericup 500mL PES 0.2μm (Express Plus) bottle-top filters for each 10 L prep of liquid media. Note that these prices include institutional discounts for some components that may or may not apply to you. I can send you our protocols if you are interested. -Tom --------------------------------------------------- Thomas Cleveland Leahy Lab Biophysics & Biophysical Chemistry Department Johns Hopkins University School of Medicine On Mon, Jun 7, 2010 at 7:18 AM, Chris Ulens <chris.ul...@med.kuleuven.be> wrote: > Hi, > I would like to get some feedback on the type of media that people are using > for insect cell culturing. > We have happily made a transition last year from using Invitrogen's SFM > medium and cellfectin to Insect-Xpress (Lonza) and polyethyleneimine for > transfection. We are moving several protein targets to large-scale cultures > and would consider cost-cutting alternatives. For example, Invitrogen and > Thermo Sci both offer media in powder form at an attractive price. Has > anyone made a systematic comparison of these media? Any particular > recommendations regarding powder media? > > Thanks. > -Chris > > --------------------------------------------------- > Chris Ulens, Ph.D. > Lab of Structural Neurobiology > Department of Molecular Cell Biology > Campus Gasthuisberg, ON1 > Herestraat 49, PB 601 > B-3000 Leuven > Belgium > e chris.ul...@med.kuleuven.be > t +32 16 345812 > f +32 16 345699 > w http://www.xtal.be >
