Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Eleanor Dodson
What is the cell difference? Space group difference- surely not??? Between the two data sets. At lowish resolution the maps can tolerate more cel difference of course than at high resolution.. Eleanor On Wed, 21 Nov 2018 at 13:09, Almudena Ponce Salvatierra < maps.fa...@gmail.com> wrote: > Dear

Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Almudena Ponce Salvatierra
Dear Pavel, thanks for the quick reply. Indeed, when I place the ligand where I suspect it could be, the program says: The polder map is likely to show the ligand. Indeed I see a blob ... I watched the video tutorial some time ago. It's very easy to use, indeed. Concerning the Fobs-Fobs maps, th

Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Pavel Afonine
Hi Almudena, I wonder to which extent I can trust the positive blobs or polder maps that > I am generating... > the answer to this question is given in corresponding paper that describes Polder map: http://journals.iucr.org/d/issues/2017/02/00/ba5254/ba5254.pdf Based on the analysis described in

[ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Almudena Ponce Salvatierra
Dear all, I have recently determined the structure of a protein for which useful data extends to 4.2 angstroms (as assessed by paired refinement after pdb redo), with some dazzling 80% solvent content and... I am expecting to find a 26-atom ligand bound to it. However the "blobbyness" of the maps