Dear all, I have recently determined the structure of a protein for which useful data extends to 4.2 angstroms (as assessed by paired refinement after pdb redo), with some dazzling 80% solvent content and... I am expecting to find a 26-atom ligand bound to it. However the "blobbyness" of the maps at this resolution doesn't help and... at the same time I wonder to which extent I can trust the positive blobs or polder maps that I am generating...
Anybody that can make other suggestions concerning how to validate the ligand binding site and/or generate other maps that could be more helpful? Any ideas will be very very welcome! Thank you very much in advance. Best wishes, Almudena ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
