Hi
you can also try dynamic light scattering of different fraction of the
peak.it will help you to know the exact molecular size of individual
peak..glutaraldehyde crosslinking is also a good option..increasing the
concentration of protein may shift the equilibrium in the direction of
higher mole
Hi,
Try glutaraldehyde crosslinking of peaks you are obtaining from SEC
individually and see if it gives you some idea.
Best wishes
Gauri
Multi-Angle Light Scattering (MALS) coupled with an HPLC column attached to
an analytical SEC column is my standard "go-to" method for determining
molecular weights/oligomeric states of peaks from SEC runs. I've only ever
used the detectors from Wyatt (
http://www.wyatt.com/solutions/hardware/hard
Hello all,
I am working with a protein that is probably a hexamer based on homology
with other proteins but when I ran it on an analytical size gel filtration
column, I see multiple peaks. I would like to determine the exact
oligomerization state of the mixture and have considered blue native gel
