Anita,
To answer a lot of your questions, you need to provide some more information
about your protein, at least some of its biophysical characteristics (size,
oligomeric state, an integral membrane protein, etc.). Just because you are
using detergents does not mean all your problems are deter
@JISCMAIL.AC.UK] On
Behalf Of anita p
Sent: Sunday, May 06, 2012 1:46 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] detergent or protein
Hi All,
Thanks for your advices. I tried staining with izit dye, please
have a look at the attached image
Hi Anita,
One question that comes up immediately is why you are using DDM @ 5%
concentration. If it's a typo, understandable. I figure most of us use DDM at
0.05-0.1% concentration, and I've used as low as 0.015%.
Also, as others have pointed out, until you shoot it, you don't know. But if
i
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] detergent or protein
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Dear Anita,
you cannot have a definite answer until to shoot the crystal and
determine its cell.
Regards,
Tim
On 05/04/12 14:48, anita p wrote:
> Hi All, I would like to have your expert adv
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Dear Anita,
you cannot have a definite answer until to shoot the crystal and
determine its cell.
Regards,
Tim
On 05/04/12 14:48, anita p wrote:
> Hi All, I would like to have your expert advice on crystals. I am
> using detergents as 5% (w/v) of DDM
Hi All,
I would like to have your expert advice on crystals.
I am using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein +
condition + detergent). The precipitant is 28% peg 20K
After 1 day I am able to see little plates of irregular shape .
I am able to see some needles if I change into MEGA
