Hi Anita, One question that comes up immediately is why you are using DDM @ 5% concentration. If it's a typo, understandable. I figure most of us use DDM at 0.05-0.1% concentration, and I've used as low as 0.015%.
Also, as others have pointed out, until you shoot it, you don't know. But if it's hexaganol with very smooth/non-sharp edges, many of us have seen it using DDM. -john On May 4, 2012, at 7:48 AM, anita p wrote: > Hi All, > I would like to have your expert advice on crystals. > I am using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein + condition > + detergent). The precipitant is 28% peg 20K > After 1 day I am able to see little plates of irregular shape . > > I am able to see some needles if I change into MEGA-10. > > I am able to see some hexagonal 3D crystals when I change the precipitant to > Ammonium sulphate and the detergent to Anapoe-C10E6 > > Is there a possibility to check whether this crystals are protein or of > detergent, even before shooting it ? > Does the control drop test help ?? > > awaiting for feedback > Anita
