Re: [ccp4bb] detergent concentration used for membrane protein purification

2007-12-21 Thread Das, Debanu
board [mailto:[EMAIL PROTECTED] On Behalf Of Rongjin Guan Sent: Friday, December 21, 2007 11:09 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] detergent concentration used for membrane protein purification Dear All sorry for a non-ccp4 related question. what is the typical concentration of deter

Re: [ccp4bb] detergent concentration used for membrane protein purification

2007-12-21 Thread Van Den Berg, Bert
To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] detergent concentration used for membrane protein purification Dear All sorry for a non-ccp4 related question. what is the typical concentration of detergents used in membrane protein purification? Is it usually within 1-10 times of CMC? My collab

[ccp4bb] detergent concentration used for membrane protein purification

2007-12-21 Thread Rongjin Guan
Dear All sorry for a non-ccp4 related question. what is the typical concentration of detergents used in membrane protein purification? Is it usually within 1-10 times of CMC? My collaborator was using 10% DDM in purifying a membrane protein. I suggested to reduce it and now he is working wit