I guess you already run SDS-PAGE to check the pellets before and after
sonication. Not only the media.
On Tue, Mar 27, 2012 at 7:35 AM, rana ibd wrote:
> Dear all
> I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB
> madia, I am having problems with the expression which sho
When you lyse the cells and spin down cellular debris, is the pellet large
and white (indicating inclusion bodies)? Is your protein soluble or
membrane? What temperature did you use for expression? What vector are you
using? Providing more details allows us to better answer your questions.
Off the
Dear all
I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB madia,
I am having problems with the expression which shows small amount of the
protein , I also have problems with
purification using NI-NTA by also having small amount even after
extensive buffer exchange , Is i
