I guess you already run SDS-PAGE to check the pellets before and after sonication. Not only the media.
On Tue, Mar 27, 2012 at 7:35 AM, rana ibd <rna19792...@yahoo.com> wrote: > Dear all > I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB > madia, I am having problems with the expression which shows small amount of > the protein , I also have problems with purification using NI-NTA by also > having small amount even after extensive buffer exchange , Is it likely due > to the small amount of protein in the medium , should I use a different kind > of media, any sugestions or any kind of details or a paper that might help I > will be thankful > > Best Regards > Rana -- Kevin Jin Candle always burns itself out to light up the tunnel ..... Website: http://www.jinkai.org/
