May I add the comment that despite the fact that it would be great if all of
the samples that I worked with would be stable at room temperature for 3 weeks,
if I had applied that as a criterion for crystallization screening and
subsequent structure determination, then my publication record would
An excellent review on improving diffraction that has not been mentioned
yet is: https://doi.org/10.1107/S0907444905032130
As for how often it happens? At my beamline we do see this fairly
infrequently, but often enough that it no longer surprises me. I suppose
that brings some comfort to the
Hi Careina
Expanding on what Tim says, try crushing your crystals to make a seed
stock, and adding it to a few *random screens - *preferably screens that
you have already tried with this target.
Search for instructions for MMS or rMMS online.
Good luck,
Patrick
On 14 August 2018 at 11:34, Ti
L.AC.UK
Betreff: [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals I have ever seen and they don't diffract at
all. Not poor diffraction, NO diffraction. Anyone know why this could be and
how I can go about fixing it? I had three beautiful crystals and no
Yes indeed!
Jon
--
Jon Hughes
(+49/0)1757929098
Sent without the use of Apple products.
Daniel M. Himmel, Ph. D. schrieb
Dear JL,
Years ago, this was a common problem when I was crystallizing myosin
constructs for my doctoral work. Some of the most beautiful crystals I
got showed lit
Dear JL,
Years ago, this was a common problem when I was crystallizing myosin
constructs for my doctoral work. Some of the most beautiful crystals I
got showed little or no diffraction. Often this occurs when there is a very
large water content in the asymmetric unit and in proteins that have a
te
a few beamlines offer this possibility these days.
best
jon
*Von:*CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag
von *Careina Edgooms
*Gesendet:* Dienstag, 14. August 2018 11:59
*An:* CCP4BB@JISCMAIL.AC.UK
*Betreff:* [ccp4bb] crystals that dont diffract :( :(
I got the most
Hi
Did you try them at room temp, in situ (straight in the plate). We
observe that time to time on our beamline, when just harvesting, not
mentioning cryo protection, is enough to loose all diffraction. It-s
rare but happens.
Regards
JL
On 14/08/2018 11:58, Careina Edgooms wrote:
I got the
Hey Careina
I had once experience the same phenomenon. No diffraction at all. That did
not make any sense to me. So i dipped the "empty" loop in the water, and
flash froze the loop in liquid nitrogen. Once exposed to X-ray, there was
no ice rings... Eventually, it turned out that system had a glit
>> medium before getting worried!
>> If they don’t diffract at RT, they are very very unlikely to diffract at
>> cryotemperatures, whatever you do to them before hand!
>> Best wishes
>> Elspeth
>>
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Be
t;
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
> *Hughes,
> Jon
> *Sent:* 14 August 2018 11:11
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(
>
> maybe it's the cryobuffer that's
, 2018 7:58 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals I have ever seen and they don't diffract at
all. Not poor diffraction, NO diffraction. Anyone know why this could be and
how I can go about fixing it? I had thre
before hand!
> Best wishes
> Elspeth
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes,
> Jon
> Sent: 14 August 2018 11:11
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(
>
> maybe
Dienstag, 14. August 2018 11:59
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals I have ever seen and they don't diffract at
all. Not poor diffraction, NO diffraction. Anyone know why this could be and
how I can go about fi
Dear Careina,
you could use the old crystals, that did not diffract, for microseeding
to regrew nicer crystals. Once you have them, try to use them as quickly
as possible. Three weeks can be a long time for crystals.
Storage in liquid nitrogen should not be the problem.
Best,
Tim
On 08/14/2018
r this possibility
these days.
best
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]Im Auftrag von Careina
Edgooms
Gesendet: Dienstag, 14. August 2018 11:59
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals
@JISCMAIL.AC.UK] On Behalf Of Hughes,
Jon
Sent: 14 August 2018 11:11
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(
maybe it's the cryobuffer that's the problem (you didn't mention it). you could
try to fish the crystals with minimal liq
p): quite a few beamlines offer this possibility
these days.
best
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Careina
Edgooms
Gesendet: Dienstag, 14. August 2018 11:59
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] crystals that dont diffract :( :(
I got the most beaut
Hello Careina,
Please send pics or didn't happen.
Anyway, in my very short experience, ugly crystals can diffract better than
beautiful ones. But have you checked first if they were protein crystals
or not?
Best of luck,
Nikk
On Tue, 14 Aug 2018, 11:59 Careina Edgooms, <
02531c126adf-dmarc
I got the most beautiful crystals I have ever seen and they don't diffract at
all. Not poor diffraction, NO diffraction. Anyone know why this could be and
how I can go about fixing it? I had three beautiful crystals and not one
diffracted. I did leave them in the drop for about 3 weeks before ha
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