Hi Careina Expanding on what Tim says, try crushing your crystals to make a seed stock, and adding it to a few *random screens - *preferably screens that you have already tried with this target.
Search for instructions for MMS or rMMS online. Good luck, Patrick On 14 August 2018 at 11:34, Tim Gruene <tim.gru...@psi.ch> wrote: > Dear Careina, > > you could use the old crystals, that did not diffract, for microseeding > to regrew nicer crystals. Once you have them, try to use them as quickly > as possible. Three weeks can be a long time for crystals. > > Storage in liquid nitrogen should not be the problem. > > Best, > Tim > > > On 08/14/2018 11:58 AM, Careina Edgooms wrote: > > I got the most beautiful crystals I have ever seen and they don't > > diffract at all. Not poor diffraction, NO diffraction. Anyone know why > > this could be and how I can go about fixing it? I had three beautiful > > crystals and not one diffracted. I did leave them in the drop for about > > 3 weeks before harvesting and in liquid nitrogen for about a month > > before diffracting. Could that be a factor? If I regrew more beautiful > > crystals and diffracted straight away could that help? > > Careina > > > > ------------------------------------------------------------------------ > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > -- > -- > Paul Scherrer Institut > Tim Gruene > - persoenlich - > OSUA/204 > CH-5232 Villigen PSI > phone: +41 (0)56 310 5297 > > GPG Key ID = A46BEE1A > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
