l.com<mailto:monica.mitta...@gmail.com>]
Sent: Wednesday, October 15, 2014 2:13 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] crystallization with hydrophobic ligands
Dear All
Can anyone give suggestions for handling the solubility problem of
hi
SCMAIL.AC.UK>
Subject: [ccp4bb] crystallization with hydrophobic ligands
Dear All
Can anyone give suggestions for handling the solubility problem of highly
hydrophobic compounds, during co-crystallization or inhibition assays?
The ligands I am using are almost insoluble in aquous medium. In DMSO or 95%
letin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Monica Mittal
[monica.mitta...@gmail.com]
Sent: Wednesday, October 15, 2014 2:13 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystallization with hydrophobic ligands
Dear All
Can anyone give suggestions for handling the solubility problem of h
Monica,
I struggled with this a lot too. I had some success with low molecular
weight PEG (100-400) as a solvent, its well worth a try.
-bob
On Wed, Oct 15, 2014 at 9:13 AM, Monica Mittal
wrote:
> Dear All
>
> Can anyone give suggestions for handling the solubility problem of
> highly hydrophob
luck, Bert
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Monica Mittal
[monica.mitta...@gmail.com]
Sent: Wednesday, October 15, 2014 2:13 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystallization with hydrophobic ligands
Dear All
Can anyone give suggestions for ha
Dear All
Can anyone give suggestions for handling the solubility problem of
highly hydrophobic compounds, during co-crystallization or inhibition
assays?
The ligands I am using are almost insoluble in aquous medium. In DMSO
or 95% Ethanol, the solubility is higher.
Besides crystallization, this so
