I agree to this last suggestion. For one of my protein, I had to add at
least 10 % of glycerol in my buffers to keep my protein stable while
purifying. For crystallization, I diluted it to 20 % glycerol (vapour
diffusion method) which allowed it to be stable in the crystallization
drop and eventu
Sorry - this may have been mentioned previously, but have you tried banging in
some glycerol (5-10%)?
J
shivesh kumar <[EMAIL PROTECTED]> wrote:>
> Dear all
> I welcome all the suggestions regarding my crystals which is coming with
> the
> precipitation.The pI of the protein is 4.2 and the drop
Dear all
I welcome all the suggestions regarding my crystals which is coming with the
precipitation.The pI of the protein is 4.2 and the drop precipitates with
MPD as low as 30% within 4-5 hrs.I am trying the pH ranging from 3.6-5.2 at
16C.There is no precipitation at 20% of MPD.Also,at high conc,t
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystal with precipitation
Hi Shivesh
I had something like this recently and found that changing the pH
stopped
the precipitation and the size of the crystals improved. If the
crystals
are large enough even if there is ppt present I would still try and
collect data on them
Hi Shivesh
I had something like this recently and found that changing the pH stopped
the precipitation and the size of the crystals improved. If the crystals
are large enough even if there is ppt present I would still try and
collect data on them. This is likely not the case for you or you wouldn
AC.UK
Subject: [ccp4bb] crystal with precipitation
Dear all
I am trying to crystallize a 7kDa protein using MPD as a precipitant at
16C.I have got small florets at 55-65% of MPD in 3-4 days.The problem is
that the drop is precipitating in one day only and the crystals are
coming
with precipitation.I
Dear all
I am trying to crystallize a 7kDa protein using MPD as a precipitant at
16C.I have got small florets at 55-65% of MPD in 3-4 days.The problem is
that the drop is precipitating in one day only and the crystals are coming
with precipitation.I have added 5% glycerol and 100mM of Nacl as an ad
