Sorry - this may have been mentioned previously, but have you tried banging in some glycerol (5-10%)?
J shivesh kumar <[EMAIL PROTECTED]> wrote:> > Dear all > I welcome all the suggestions regarding my crystals which is coming with > the > precipitation.The pI of the protein is 4.2 and the drop precipitates with > MPD as low as 30% within 4-5 hrs.I am trying the pH ranging from 3.6-5.2 > at > 16C.There is no precipitation at 20% of MPD.Also,at high conc,the protein > precipitates.The crystals are coming as florets as I mentioned with > 2+2.Should I go for higher pH like uptp 8.5 or so.crystals are not > growing > bigger in size so that I can mount.Should I try changing the > temperature.Ican use 4C and > 16C.Right now I am keeping plates at 16C.I am concentrating the protein > through lyophilization.As soon I pool it for Gel filtration,majority of > it > get precipitated,not soluble protein.Is there any method to make the > protein > stable? > I appreciate all the informative suggestions.Thanx in advance and thanx > everybody who has suggested the various options. > Shivesh kumar -- Professor James Whisstock NHMRC Principal Research Fellow / Monash University Senior Logan fellow Department of Biochemistry and Molecular Biology Monash University, Clayton Campus, PO Box 13d, VIC, 3800, Australia +613 9905 3747 (Phone) +613 9905 4699 (Fax) +61 418 170 585 (Mobile)
