oard on behalf of Christoph Best
> Sent: Tue 7/13/2010 10:20 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] conversion of cyroEM reconstruction from
> MRC to CCP4 format
>
> Hello,
>
> we have found that
the original poster could make their problem maps available??
>
> Cheers
> Martyn
>
> -Original Message-
> From: CCP4 bulletin board on behalf of Christoph Best
> Sent: Tue 7/13/2010 10:20 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] conversion of cyro
version of cyroEM reconstruction from MRC to CCP4
format
Hello,
we have found that there are some minor issues in MRC files using in Em
that can make them unpalatable to the CCP4 map library. You can easily
look at header fields in Python - here is a sample program to quickly
check some of the m
Hello,
we have found that there are some minor issues in MRC files using in Em
that can make them unpalatable to the CCP4 map library. You can easily
look at header fields in Python - here is a sample program to quickly
check some of the more common problems.
from struct import *
header=
Hello James,
You can try the map2map from the SITUS package (
http://situs.biomachina.org/fguide.html) or em2em (
http://www.imagescience.de/em2em/)
Cheers
Filip Van Petegem
On Tue, Jul 13, 2010 at 5:32 AM, James Whittle
wrote:
> Hi all-
>
> I'm trying to convert a cryoEM map from FREALIGN f
It is possible that the map is just too big. You might run into
"problems" with file I/O in general if it is bigger than 2 GB. If you
have a 64-bit machine with CCP4 completely re-compiled in 64-bit mode,
then it "should" be able to read the large files (but I have not had
much luck with this
Hi James,
I had similar problems with MRC maps. As a solution, a colleague of
mine, working with EM, read the MRC map into SPIDER and saved it as a
CCP4 map. That worked for me. Unfortunately, my colleague is absent for
some time, and I'm still not familiar with EM software. But maybe, you
f
Hi all-
I'm trying to convert a cryoEM map from FREALIGN for use with various CCP4
programs, or with MAPMAN. Even though the MRC format is derived from the
CCP4 map file format, every program I've tried fails to open it, (except for
Chimera which displays the map but cannot save it to another form
