Hi James,
I had similar problems with MRC maps. As a solution, a colleague of
mine, working with EM, read the MRC map into SPIDER and saved it as a
CCP4 map. That worked for me. Unfortunately, my colleague is absent for
some time, and I'm still not familiar with EM software. But maybe, you
find a colleague at your institute to help you with that.
Good luck,
Dirk.
Am 13.07.10 14:32, schrieb James Whittle:
Hi all-
I'm trying to convert a cryoEM map from FREALIGN for use with various
CCP4 programs, or with MAPMAN. Even though the MRC format is derived
from the CCP4 map file format, every program I've tried fails to open
it, (except for Chimera which displays the map but cannot save it to
another format for lack of memory).
For example, in MAPMASK I get the error:
>>>>>> CCP4 library signal ccp4_map:No associated header (Error)
raised in ccp4_cmap_open <<<<<<
>>>>>> CCP4 library signal ccp4_map:Cannot open file (Error)
raised in MRDHDS <<<<<<
or with MAPMAN:
**CCPOPN ERROR** FORMATTED OLD file open failure on unit 1
Logical name: DLP_100.map, File name: DLP_100.map
Cannot send after transport endpoint shutdown
ERROR --- While reading map header. Sorry !
ERROR --- While opening map file
Is there a way to re-write the header to be compatible with CCP4?
--James
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Dirk Kostrewa
Gene Center Munich, A5.07
Department of Biochemistry
Ludwig-Maximilians-Universität München
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Germany
Phone: +49-89-2180-76845
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