Re: [ccp4bb] calc SF from map for MR - PHASER

2008-10-15 Thread Eleanor Dodson
It is tricky, but maps can work. My procedure was: Extend map file to P1 Put a dummy "atom" at the position you feel is about in the centre of the molecule density. Make a mask around this atom with a radius of nA which you feel is big enough to cover most of your molecule. Use this mask to m

Re: [ccp4bb] calc SF from map for MR - PHASER

2008-10-14 Thread Edward A. Berry
Heidi Schubert wrote: Hi I would like to convert a map into SFs to use for molecular replacement. Read on if you think you can help. I have a 3.7A MAD data set and a few 30% similar homology models for crystal in i222 space group. The combination of the two pieces of information generated

[ccp4bb] calc SF from map for MR - PHASER

2008-10-14 Thread Heidi Schubert
Hi I would like to convert a map into SFs to use for molecular replacement. Read on if you think you can help. I have a 3.7A MAD data set and a few 30% similar homology models for crystal in i222 space group. The combination of the two pieces of information generated solvent flattened phases