Hi,
we currently use Amicon Ultra with some proteins, but with some others we
also have seen precipitation problems.
In those cases we were happy with Vivacon (from Sartorius as well). They are
thought for DNA but they
work also with proteins. They resemble the Centricon devices which were
previous
Hello Jan,
I've used both with soluble and membrane proteins happily. I haven't
seen significant differences with protein behavior or performance in one or
the other, but of course, your favorite protein may differ. I've found that
buffer condition is a more important variable than membrane t
The vivaspins are the best thing in
ultrafiltration since sliced bread. I learned about them while I
was at the NIH several years ago and haven't looked back. Fast and
low-binding. PES membranes are superior for minimizing protein
binding, and are also less subjec
Hi Jan,
In our lab we use both the Amicon Ultras and the Vivaspins (soluble and
membrane proteins).
What I can tell you is that me and a few other colleagues had the
experience (and still do) of specific soluble proteins precipitating on
the Cellulose membrane
of the Amicon. By switching to
Hi Jan
I have used Vivaspins for membrane proteins and they generally work fine. The
exact pore size might be different though between Vivaspin and Amicon with the
same specifications, say e.g. 100,000 MWCO. So you might find that your protein
is retained in one of them, while it is in the flow
Hi Jan,
I've used both, and I've used them on membrane binding proteins,
extracellular matrix proteins and intracellular proteins.
I know of some cases where protein loss is very severe, but that varies on a
case-by-case basis. A general observation is that proteins with which losses
are bad, are
Dear all,
sorry, for the slightly off-topic theme, but I wonder if anyone has
compared the above mentioned ultracentrifugation devices, thoroughly.
Currently, we are using the Amicon Ultra, but as the Vivaspins are
considerably cheaper we are considering to change.
I used both with extracellula
