Hi, we currently use Amicon Ultra with some proteins, but with some others we also have seen precipitation problems. In those cases we were happy with Vivacon (from Sartorius as well). They are thought for DNA but they work also with proteins. They resemble the Centricon devices which were previously produced by Millipore.
Claudia 2011/10/6 Ho Leung Ng <h...@hawaii.edu> > Hello Jan, > > I've used both with soluble and membrane proteins happily. I haven't > seen significant differences with protein behavior or performance in one or > the other, but of course, your favorite protein may differ. I've found that > buffer condition is a more important variable than membrane type. > > > Ho > > Ho Leung Ng > University of Hawaii at Manoa > Assistant Professor, Department of Chemistry > holeung...@hawaii.edu > -- Claudia Binda University of Pavia Dept. Genetics and Microbiology via Ferrata 1, 27100 Pavia - Italy Phone: +39-0382-985535 Fax: +39-0382-528496 E-mail: claudia.bi...@unipv.it Web: http://www.unipv.it/biocry
