Hi Gurudatt,
If the alignment of the tilt series is already good enough (this also applies
to .ali stacks from IMOD), you can run eman2 tomogram reconstruction using the
—noali and —niter=0 options, which will just reconstruct your tilt series in
their current alignment. Note that the tilt axis
Dear All,
I was wondering if we can use the aligned tilt series that we get from
Serial EM as raw data directly for reconstruction and particle picking in
EMAN2.
Thank you so much in advance.
Sincerely,
Gurudatt
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