SDS is preferred for fast transfer.
On Sat, Mar 21, 2015 at 4:24 PM, rohit kumar wrote:
> thanks for the suggestion,
> is SDS will help in case of CAPS buffer?
> molecular weight of my protein is 40 kda.
> or can i use PO4 buffer for N-ter sequencing.
>
> On Sat, Mar 21, 2015 at 12:42 PM, Anirba
thanks for the suggestion,
is SDS will help in case of CAPS buffer?
molecular weight of my protein is 40 kda.
or can i use PO4 buffer for N-ter sequencing.
On Sat, Mar 21, 2015 at 12:42 PM, Anirban Kundu
wrote:
> Try with 5 % methanol along with CHAPS buffer and the membrane should be
> methanol
Try with 5 % methanol along with CHAPS buffer and the membrane should be
methanol charged
On Fri, Mar 20, 2015 at 10:48 PM, rohit kumar wrote:
> Dear all,
>
> sorry for off topic discussion.
> I am having trouble transferring protein onto PVDF membrane for N-terminal
> sequencing. When I used Tr
Dear all,
sorry for off topic discussion.
I am having trouble transferring protein onto PVDF membrane for N-terminal
sequencing. When I used Tris-Glycine-Methanol in transfer buffer I got good
blot, but when I used CAPS buffer (pH 11 set with 2N NaOH and 10% methanol)
I did not get any blot. I tri
