Dear all, sorry for off topic discussion. I am having trouble transferring protein onto PVDF membrane for N-terminal sequencing. When I used Tris-Glycine-Methanol in transfer buffer I got good blot, but when I used CAPS buffer (pH 11 set with 2N NaOH and 10% methanol) I did not get any blot. I tried transferring with variable current and durations (2hrs to overnight). Can anyone suggest what I might do??
-- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
