Re: [ccp4bb] Too many microcrystals.......

Hi Dilip- Try using your microcrystals as seeds and try the matrix microseeding method: Acta Crystallogr D Biol Crystallogr.2007 Apr;63(Pt 4):550-4. Epub 2007 Mar 16. An automated microseed matrix-screening method for protein crystallization. D'Arcy A

Re: [ccp4bb] Too many microcrystals.......

: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Too many microcrystals... hi guys I am trying to crystallize protein complexed with peptide(14mer). In initial screening i got microcrystals in following condition - 0.2M MgCL2 0.1M Tris pH 8.5 28 % PEG 4000 Ptotein concentration - 12.5mg/ml and

Re: [ccp4bb] Too many microcrystals.......

Hello Dilip Badgujar, What exactly do you mean by "not working" when you lowered to concentration? Did you lower the complex concentration and at some point you got - no precipitation at all - no crystals but aggregate precipitation - something else? You also do not mention that you tried loweri

Re: [ccp4bb] Too many microcrystals.......

Sure it's not the peptide that crystallizes ? You could try adding glycerol, exchanging the PEG, lowering the PEG playing with the pH or changing to a different buffer system. You might have done all these already, then you could also try a larger drop size. You could also mix your protein with yo

[ccp4bb] Too many microcrystals.......

hi guys I am trying to crystallize protein complexed with peptide(14mer). In initial screening i got microcrystals in following condition - 0.2M MgCL2 0.1M Tris pH 8.5 28 % PEG 4000 Ptotein concentration - 12.5mg/ml and peptide conc.-5mg/ml Complex ratio- (Protein-Peptide)(1:1.5) Incubation