It seems likely that you could even monitor the heme group somehow, depending
on its
characteristics. I would bet that when the protein denatures, the
released/exposed heme's
spectroscopic properties change in some way. I would just see what happens with
the heme itself...
Jacob
==
Dear all,
To optimize my crystallization conditions I would like to do thermal melts but
unfortunately, there is heme in the molecule which messed up my experiments.
The dye I used is colored orange ("SYPRO orange"). Has anybody an idea if it is
possible to get serious results with an appropria
