Dear all, To optimize my crystallization conditions I would like to do thermal melts but unfortunately, there is heme in the molecule which messed up my experiments. The dye I used is colored orange ("SYPRO orange"). Has anybody an idea if it is possible to get serious results with an appropriate dye instead or does Thermal melt never work with heme in the protein? Many thanks!
Jan --------------------------------- Die etwas anderen Infos rund um das Thema Reisen. BE A BETTER WELTENBUMMLER!