You can put your "dogbowl dewar" under the microscope and focus it on
the surface of the liquid nitrogen. This works very well once you have
got the lighting right. You want to shine the light on the bottom of
the dewar so that it acts as a backlight for your loop. You can hold
the loop just
Checking Cryo-Conditions under the microscope can actually be very
useful. Even if you have access to a X-ray beam, simple pre-tests can
be very informative.
This can be done as follows:
A 24well plate cover is placed on a stereo microscope open side down,
a second one on top of it the oth
I think I posted this link maybe two years ago:
http://idb.exst.jaxa.jp/db_data/protein/search-e.php?
Jürgen
On Sep 22, 2009, at 12:48 PM, Elspeth Garman wrote:
or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
Good luck
EFG
Paul Leonard wrote:
Hi Claudia
It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in hous
Hi Claudia
It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in house you could look at the
hampton crystallization screen conditions (or another supplier). Find a
condition with a similar amount and type of precipitant as you have in
your
It might be risky (and silly), but I have done this successfully. I
just put a small dewar of liquid N2 under the scope, focussed
directly above the surface and looked to see if cryo solutions froze
clear or not. The ones that did worked out when we got to the
beamline (we'd verified that
Hi Claudia
"By eye" is not a bad approximation. The trick I learnt at the JCSG (I
forget the originator, sorry) is to suck up some solution in a P2
pipette tip (the clear ones), dip it in lN2, and then hold it just above
the lN2 (where it's still cold) to check for clearness or not.
Is a go
Dear Claudia,
please be so kind and tell us all what is / are the crystallization
condition(s)? It would help to us to answer your question!
- J. -
Claudia Scotti wrote:
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostre
I have not heard of anyone checking for suitable cryo in that fashion how
fast can you do it before it is affected by room temp and how would you know
about the ice rings, but when you have crystal(s) and not crystal you can
freeze different ones in several different cryos (sugars, glycerol etc) an
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostream?
I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is it
enough to try to freeze the cryoconditions in liquid nitrogen checking under
the mi
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