Re: [ccp4bb] Suggestion for storage buffer for protein

2012-08-20 Thread Anna Gardberg
Hi Christine, I'm just now catching up on some of your older posts, so this may be too late too help. For Fab crystallization, my successful protein stabilization buffer contained 20-50 mM MES pH 6.0 or 6.5 with the minimum possible amount of salt to stabilize the protein - 50 mM NaCl, perhaps. Low

[ccp4bb] Suggestion for storage buffer for protein

2012-05-04 Thread Harman, Christine
Hi All, I was wondering if any of you could recommend a buffer to store my Fab fragment for crystallization trials. My Fab is currently stored in 0.1M Sodium Acetate pH5, 150mM NaCl and I have had some success with crystallization hits (three conditions that have actually single crystals); howe