edge leads to average
spot intensities being reduced by a factor of 8.
Thanks!
Colin
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dirk
Kostrewa
Sent: 11 January 2012 09:15
To: ccp4bb
Subject: Re: [ccp4bb] Sub-angstrom resolution
Dear
On 11 Jan 2012, at 11:36, Thomas Womack wrote:
>
> On 11 Jan 2012, at 02:13, Artem Evdokimov wrote:
>
>> There are two sides to this qustion: the scientific one is actually easier
>> to answer in generic terms - but I also would like to point out the very
>> recent example of a mystery that re
On 11 Jan 2012, at 02:13, Artem Evdokimov wrote:
> There are two sides to this qustion: the scientific one is actually easier to
> answer in generic terms - but I also would like to point out the very recent
> example of a mystery that required very high resoluton (and orthogonal
> techniques)
Dear Colin,
Am 10.01.12 18:08, schrieb Colin Nave:
3. The structure factors are lower for large unit cells. This will mean they
will be harder to detect, particularly if there is a high background.
But aren't the total structure factors of a unit cell the sum of the
atomic structure fact
There are two sides to this qustion: the scientific one is actually easier
to answer in generic terms - but I also would like to point out the very
recent example of a mystery that required very high resoluton (and
orthogonal techniques) to answer, namely the puzzle of the light atom in
the center
One more to add to Ed's list:
highly accurate ultra-high resolution structures may allow calculation of
electrostatic potential around protein ligand binding site. There are
plenty of reference...
Pavel
On Tue, Jan 10, 2012 at 11:04 AM, Ed Pozharski wrote:
> On Tue, 2012-01-10 at 18:30 +, T
On Tue, 2012-01-10 at 18:30 +, Theresa H. Hsu wrote:
> Thank you for the interesting replies so far.
>
> Please let me ask a related question - at what resolution should we stop
> efforts to get better diffracting crystals? Are there *biological* questions
> that a model with 1.8-2.0 A resol
Thank you for the interesting replies so far.
Please let me ask a related question - at what resolution should we stop
efforts to get better diffracting crystals? Are there *biological* questions
that a model with 1.8-2.0 A resolution (with combination of complementary
methods like spectroscopy
Colin
-Original Message-
From: Ed Pozharski [mailto:epozh...@umaryland.edu]
Sent: 10 January 2012 16:09
To: Nave, Colin (DLSLtd,RAL,DIA)
Cc: ccp4bb
Subject: Re: [ccp4bb] Sub-angstrom resolution
On Tue, 2012-01-10 at 09:04 +, Colin Nave wrote:
> Yes, I think Ed's analysis is a bit m
I think once you start getting down to such small crystals, the spots
are not really important, as the pattern starts getting continuous.
Interestingly enough, I guess for single-molecule diffraction,
resolution is limited only by radiation damage, and not by any sort of
lattice disorder (or even b
On Tue, 2012-01-10 at 09:04 +, Colin Nave wrote:
> Yes, I think Ed's analysis is a bit misleading.
I apologize if I misled anyone. Re-reading my post, I can see that it
lacked precision. Indeed, in a perfect monocrystal all the molecules
are lined up perfectly, so I should have emphasized ra
sage-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ethan
Merritt
Sent: 09 January 2012 19:47
To: ccp4bb
Subject: Re: [ccp4bb] Sub-angstrom resolution
On Monday, January 09, 2012 11:37:23 am Ed Pozharski wrote:
> On Mon, 2012-01-09 at 18:15 +, Theresa H. Hsu wrote:
>
On Monday, January 09, 2012 11:37:23 am Ed Pozharski wrote:
> On Mon, 2012-01-09 at 18:15 +, Theresa H. Hsu wrote:
> > Dear crystallographers
> >
> > A theoretical question - can sub-angstrom resolution structures only be
> > obtained for a limited set of proteins? Is it impossible to achieve
On Mon, 2012-01-09 at 18:15 +, Theresa H. Hsu wrote:
> Dear crystallographers
>
> A theoretical question - can sub-angstrom resolution structures only be
> obtained for a limited set of proteins? Is it impossible to achieve for
> membrane proteins and large complexes?
>
> Theresa
On the ma
The word "theory" in this thread/question has to be clarified better.
Jacob
On Mon, Jan 9, 2012 at 1:27 PM, Tim Gruene wrote:
> -BEGIN PGP SIGNED MESSAGE-
> Hash: SHA1
>
> Hi,
>
> in my opinion the resolution limit of crystals from large complexes/
> membrane proteins is more likely due
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Hi,
in my opinion the resolution limit of crystals from large complexes/
membrane proteins is more likely due to lattice imperfections and
long-range disorder, and cold neither cold temperatures nor stronger
radiation sources would circumvent this pro
In theory, no: sub-angstrom resolution can be obtained for any and all
proteins, including membrane proteins, and for large complexes. In
reality, it becomes technically very difficult to achieve; you would need
ever-colder temperatures and ever-stronger irradiation sources.
P.S. In theory, the
Dear crystallographers
A theoretical question - can sub-angstrom resolution structures only be
obtained for a limited set of proteins? Is it impossible to achieve for
membrane proteins and large complexes?
Theresa
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