How about DNA synthesis within crystals?
1: Kiefer JR, Mao C, Braman JC, Beese LS. Visualizing DNA replication in
a catalytically active Bacillus DNA polymerase crystal. Nature. 1998 Jan
15;391(6664):304-7. PubMed PMID: 9440698.
2: Johnson SJ, Taylor JS, Beese LS. Processive DNA synthesis obs
I saw also a great domain (around 150aa) movement on one of the subunits
when crystals of the protein (yeast acetylglutamate kinase) were soaked
with its inhibitor (arginine):
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0034734
2012/10/9 Alan Cheung
> Depends on what you m
Depends on what you mean by large, but we saw very obvious domain
movement when we soaked a protein (transcription factor TFIIS) into
crystals of RNA Polymerase II (PMIDs 12914699/21346759/22726432). We
recently animated this conformational change, and you can watch it at:
http://www.youtube.
: WENHE ZHONG
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, 9 October 2012, 9:33
Subject: [ccp4bb] Structure example request for large domain movement "in
crystallo" soaking
Dear CCP4 members,
Recently, I got a ligand soaking structure to clearly show a large domain (~100
amino acids
Dear Wenhe,
One example we have is the protein kinase Nek2. We co-crystallised with ADP and
then soaked in the inhibitors. The activation loop was free to move around and
adopt a number of different conformations (e.g. to DFG-out conformation).
References:
Westwood I, Cheary D-M, Baxter JE, Ri
Dear CCP4 members,
Recently, I got a ligand soaking structure to clearly show a large domain
(~100 amino acids) movement compared to the no soaking structure. Although
there are some reported examples of this enzyme to suggest the flexibility
of this large domain which is relevant to substrate bin
