Re: [ccp4bb] Structural biology inside the cell

2009-03-13 Thread Narayanan Ramasubbu
ar...@xtals.org wrote: Hello, 1. As long as all proteins have seventy amino-acids or less and express in E. coli in mM concentrations - we're in business. 2. As for the question below - my favorite answer is 'It will take a week and ten million dollars in unmarked bills. We begin as soon as the

Re: [ccp4bb] Structural biology inside the cell

2009-03-13 Thread artem
Hello, 1. As long as all proteins have seventy amino-acids or less and express in E. coli in mM concentrations - we're in business. 2. As for the question below - my favorite answer is 'It will take a week and ten million dollars in unmarked bills. We begin as soon as the money arrives.'. Artem

Re: [ccp4bb] Structural biology inside the cell

2009-03-13 Thread Kay Diederichs
Mark J. van Raaij schrieb: ... And these wrong impressions are all too common, at least around here. As another example, more than once I have been asked by someone if they give me the sequence or name of a protein (even membrane proteins), how many days would it take us to provide them the cry

Re: [ccp4bb] Structural biology inside the cell

2009-03-13 Thread Mark J. van Raaij
Dear All, Just to clarify that I completely agree with the interest and relevance of the two papers discussed in the News & Views and with 99% of the News & Views article itself - it is just that readers from non- structural biology fields may get the wrong impression from the 2 "was" word

Re: [ccp4bb] Structural biology inside the cell

2009-03-12 Thread Felix Frolow
Dear Mark Stay calm Buzz-words come and very frequently do not stay, they go away with the artifacts they advocate... Dr Felix Frolow Professor of Structural Biology and Biotechnology Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica

[ccp4bb] Structural biology inside the cell

2009-03-12 Thread Mark J. van Raaij
Dear All, a News & Views article in Nature 458, pages 37-38 of 5 March 2009 (link below) states: "The development of structural biology WAS historically based on the principle of divide and conquer — individual proteins were purified to homogeneity and their atomic structures were solved in