Hello, 1. As long as all proteins have seventy amino-acids or less and express in E. coli in mM concentrations - we're in business.
2. As for the question below - my favorite answer is 'It will take a week and ten million dollars in unmarked bills. We begin as soon as the money arrives.'. Artem > As another example, more than once I have been asked by someone if > they give me the sequence or name of a protein (even membrane > proteins), how many days would it take us to provide them the crystal > structure. > > Mark > > Mark J. van Raaij > Dpto de Bioquímica, Facultad de Farmacia > Universidad de Santiago > 15782 Santiago de Compostela > Spain > http://web.usc.es/~vanraaij/ > > > > > > > > On 12 Mar 2009, at 21:57, Felix Frolow wrote: > >> Dear Mark >> Stay calm >> Buzz-words come and very frequently do not stay, they go away with >> the artifacts they advocate... >> >> Dr Felix Frolow >> Professor of Structural Biology and Biotechnology >> Department of Molecular Microbiology >> and Biotechnology >> Tel Aviv University 69978, Israel >> >> Acta Crystallographica D, co-editor >> >> e-mail: mbfro...@post.tau.ac.il >> Tel: ++972 3640 8723 >> Fax: ++972 3640 9407 >> Cellular: ++972 547 459 608 >> >> On Mar 12, 2009, at 6:14 PM, Mark J. van Raaij wrote: >> >>> Dear All, >>> >>> a News & Views article in Nature 458, pages 37-38 of 5 March 2009 >>> (link below) states: >>> "The development of structural biology WAS historically based on >>> the principle of divide and conquer individual proteins were >>> purified to homogeneity and their atomic structures were solved in >>> vitro by using either X-ray crystallography or nuclear magnetic >>> resonance (NMR) spectroscopy. This approach WAS tremendously >>> successful, and led to the creation of a protein-structure databank >>> that currently contains more than 50,000 structures." >>> >>> I find the past tense here too much... >>> >>> Greetings, >>> >>> Mark >>> >>> Mark J. van Raaij >>> Dpto de Bioquímica, Facultad de Farmacia >>> Universidad de Santiago >>> 15782 Santiago de Compostela >>> Spain >>> http://web.usc.es/~vanraaij/ >>> >>> http://www.nature.com/nature/journal/v458/n7234/full/458037a.html >>> Structural biology: Inside the living cell >> >
