Re: [ccp4bb] Soaking to Remove Bound Ligands from Crystals

2010-03-19 Thread Tao-Hsin Chang
Dear Critton We have done a case, which was to remove the ligand A from the crystal in complex with ligand B and metal ions. Please see the link of this case below (Methods). http://www.ncbi.nlm.nih.gov/pubmed/20139160 Cheers, Tao-Hsin

Re: [ccp4bb] Soaking to Remove Bound Ligands from Crystals

2010-03-18 Thread Anastassis Perrakis
t: [ccp4bb] Soaking to Remove Bound Ligands from Crystals Dear ccp4bb, I recently came across a thread from 2007 discussing the removal of ions/ligands from protein crystals (https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0705&L=CCP4BB&P=R35929 ). The thread cited a couple of paper

[ccp4bb] Soaking to Remove Bound Ligands from Crystals

2010-03-17 Thread Artem Evdokimov
re 11 (12), pp. 1537-1546 > > George > > > > -Original Message- > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of > Critton, David > Sent: Wednesday, March 17, 2010 6:02 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Soaking to Remove B

Re: [ccp4bb] Soaking to Remove Bound Ligands from Crystals

2010-03-17 Thread Kontopidis George
George -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Critton, David Sent: Wednesday, March 17, 2010 6:02 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Soaking to Remove Bound Ligands from Crystals Dear ccp4bb, I recently came across a thread

[ccp4bb] Soaking to Remove Bound Ligands from Crystals

2010-03-17 Thread Critton, David
Dear ccp4bb, I recently came across a thread from 2007 discussing the removal of ions/ligands from protein crystals (https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0705&L=CCP4BB&P=R35929). The thread cited a couple of papers (Ray et al. 1991; Schreuder et al. 1988) in which crystals were g