Dear ccp4bb,
I recently came across a thread from 2007 discussing the removal of
ions/ligands from protein crystals
(https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0705&L=CCP4BB&P=R35929). The
thread cited a couple of papers (Ray et al. 1991; Schreuder et al. 1988) in
which crystals were grown in the presence of a given ligand, then soaked in
conditions containing little (if any) ligand in order to remove the ligand from
these crystals.
I would like to know if there are any other reports of this method, especially
if any structures were solved from pre- and post-soaked crystals, and what
kinds of conformational changes (if any) accompany these kinds of soaks.
Thank you in advance,
David A. Critton
Graduate Student, Page Laboratory
Department of Molecular Biology, Cell Biology & Biochemistry
Brown University
Providence, RI
