Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-30 Thread James Holton
*From:* James Holton *Sent:* Thursday, August 29, 2019 10:50 AM *To:* Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using?  And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kim

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-30 Thread Green, Todd Jason
C.UK<mailto:CCP4BB@JISCMAIL.AC.UK> <mailto:CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using? And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kimberly Stanek wrote: Hi folks, We have a protein th

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-30 Thread James Holton
(949) 824-0014 *From:* James Holton *Sent:* Thursday, August 29, 2019 10:50 AM *To:* Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using?  And which beamline? -J

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-29 Thread Francis Reyes
Ah, low resolution experimental phasing. Fun stuff, seems like a lost art these days. Are the crystals large ? Is it possible to do a (continuous helical data) collection? (Manually or if your beamline permits you to). Granted this is anomalous, is it possible to then merge enough partial da

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-29 Thread graeme.win...@diamond.ac.uk
ci.edu>>; CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using? And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kimberly Stanek

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-29 Thread Kimberly Stanek
James Holton Sent: Thursday, August 29, 2019 10:50 AM To: Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using? And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kimberly Stanek wrot

Re: [ccp4bb] Semet derivative dying almost immediately in beam

2019-08-29 Thread James Holton
What exposure time are you using?  And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kimberly Stanek wrote: Hi folks, We have a protein that we have been trying to solve the structure of for a while now but so far haven't been able to get any diffraction better than ~4.5

[ccp4bb] Semet derivative dying almost immediately in beam

2019-08-29 Thread Kimberly Stanek
Hi folks, We have a protein that we have been trying to solve the structure of for a while now but so far haven't been able to get any diffraction better than ~4.5A. I was able to collect a full 360 degrees of data and index, but MR is failing so we have turned to de novo phasing. Recently we