*From:* James Holton
*Sent:* Thursday, August 29, 2019 10:50 AM
*To:* Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] Semet derivative dying almost immediately in
beam
What exposure time are you using? And which beamline?
-James Holton
MAD Scientist
On 8/29/2019 10:26 AM, Kim
C.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam
What exposure time are you using? And which beamline?
-James Holton
MAD Scientist
On 8/29/2019 10:26 AM, Kimberly Stanek wrote:
Hi folks,
We have a protein th
(949) 824-0014
*From:* James Holton
*Sent:* Thursday, August 29, 2019 10:50 AM
*To:* Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] Semet derivative dying almost immediately in beam
What exposure time are you using? And which beamline?
-J
Ah, low resolution experimental phasing. Fun stuff, seems like a lost art these
days.
Are the crystals large ?
Is it possible to do a (continuous helical data) collection? (Manually or if
your beamline permits you to).
Granted this is anomalous, is it possible to then merge enough partial da
ci.edu>>;
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam
What exposure time are you using? And which beamline?
-James Holton
MAD Scientist
On 8/29/2019 10:26 AM, Kimberly Stanek
James Holton
Sent: Thursday, August 29, 2019 10:50 AM
To: Kimberly Stanek ; CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam
What exposure time are you using? And which beamline?
-James Holton
MAD Scientist
On 8/29/2019 10:26 AM, Kimberly Stanek wrot
What exposure time are you using? And which beamline?
-James Holton
MAD Scientist
On 8/29/2019 10:26 AM, Kimberly Stanek wrote:
Hi folks,
We have a protein that we have been trying to solve the structure of
for a while now but so far haven't been able to get any diffraction
better than ~4.5
Hi folks,
We have a protein that we have been trying to solve the structure of for a
while now but so far haven't been able to get any diffraction better than
~4.5A. I was able to collect a full 360 degrees of data and index, but MR is
failing so we have turned to de novo phasing.
Recently we