ALS 822. I tried as low as 0.2 sec exposure but it didn't seem to help much.
Kimberly Stanek Postdoctoral Researcher, Goulding Lab Co-chair, UCI Postdoctoral Association University of California, Irvine Natural Science I, Room 2302 (949) 824-0014 ________________________________ From: James Holton <jmhol...@lbl.gov> Sent: Thursday, August 29, 2019 10:50 AM To: Kimberly Stanek <ksta...@uci.edu>; CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam What exposure time are you using? And which beamline? -James Holton MAD Scientist On 8/29/2019 10:26 AM, Kimberly Stanek wrote: Hi folks, We have a protein that we have been trying to solve the structure of for a while now but so far haven't been able to get any diffraction better than ~4.5A. I was able to collect a full 360 degrees of data and index, but MR is failing so we have turned to de novo phasing. Recently we prepared crystals of the Semet derivative under the same condition. While these crystals diffracted to about the same resolution, I found they were dying after just one or two snaps, even with increased beam attenuation and decreased exposure time. I am wondering if anyone has experienced anything like this before and had any suggestions on what to do about it. Thank you, Kimberly Stanek Postdoctoral Researcher, Goulding Lab Co-chair, UCI Postdoctoral Association University of California, Irvine Natural Science I, Room 2302 (949) 824-0014 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
