Hi Gloria,
Just to follow up Zhijie's email. We use E.coli expressed ULP1 protease
extensively in my lab, and it's very active and quite a bit cheaper than the
commercial version. My last prep from 2L produced greater than 50mg, and I've
more or less froze it directly off of the NiNTA elution.
bject: [ccp4bb] SUMO(ULP-1) protease
My fellow crystallographers,
We are thinking the SUMO/His vectors would be nice to have in the lab
aresenal... but. The stumbling block is that the protease needed for
cleavage is very expensive at crystallography scale. SUMO(ULP-1)
protease costs ~$700/mg
Gloria,
Why don't you introduce TEV-cleavage site? You can clone it between His-
and sumo-protein_of_interest (if you want co-crystallise sumo with your
protein) or between His-sumo and protein_of_interest (if you want to
crystallise your protein only).
Vitali
On Thu, May 24, 2012 at 2:41 PM, Gl
Gene synthesis and make your own!
Tony.
Sent from my iPhone
On 24 May 2012, at 20:42, "Gloria Borgstahl" wrote:
> My fellow crystallographers,
> We are thinking the SUMO/His vectors would be nice to have in the lab
> aresenal... but. The stumbling block is that the protease needed for
> clea
My fellow crystallographers,
We are thinking the SUMO/His vectors would be nice to have in the lab
aresenal... but. The stumbling block is that the protease needed for
cleavage is very expensive at crystallography scale. SUMO(ULP-1)
protease costs ~$700/mg fusion protein. It would not be a prob
