My fellow crystallographers, We are thinking the SUMO/His vectors would be nice to have in the lab aresenal... but. The stumbling block is that the protease needed for cleavage is very expensive at crystallography scale. SUMO(ULP-1) protease costs ~$700/mg fusion protein. It would not be a problem for labs using micrograms of protein, but is prohibitive at our level of protein purification. Has anyone found a way around this? Your pal, Gloria
- [ccp4bb] SUMO(ULP-1) protease Gloria Borgstahl
- Re: [ccp4bb] SUMO(ULP-1) protease Antony Oliver
- Re: [ccp4bb] SUMO(ULP-1) protease Vitali Stanevich
- Re: [ccp4bb] SUMO(ULP-1) protease Zhijie Li
- Re: [ccp4bb] SUMO(ULP-1) protease John K Lee