Hi Tim,
an R-factor of 0.64 does not sound like a solution. You may try submitting
your problem to our automated MR server CaspR and see if something pops up :
http://www.igs.cnrs-mrs.fr/Caspr2/
Kind regards,
Karsten.
On Monday 19 February 2007 17:15, [EMAIL PROTECTED] wrote:
> I would take
Hi Emmanuel,
try MZ protocol (MZ = multi-zone) in phenix.refine. It does the smart
rigid body refinement starting from a few low resolution reflections and
ending up with using all data. In-between it does the maximum-likelihood
bulk-solvent modeling and scaling. All together it results in a V
I would take a well-ordered helix out of your
allegedly-correctly-placed model (BEFORE doing any refinement other
than rigid body), phase a 2Fo-Fc and an Fo-Fc map with it, and see if
the helix shows up. If it does, there is some reality in your
solution. If it doesn't, start over from scratc
Just an aside to this - I have had R factors > 55% which then refine..
It is when they dont refine you are in trouble ..
Eleanor
Jiamu Du wrote:
*Dear Prata:*
First, I think you'd better check your subgroups. Maybe you got
opposite hand of the data.
Or change a model. When the Rfactor is ab
Hi,
I dare say with an R-factor of 0.64, your MR solution is simply wrong.
Just into the blue, you could
1) try various MR-programs (phaser, molrep, amore...)
2) try a different search model
3) try your best model but start with subdomains or chop off possibly
flexible parts
4) have a look at
*Dear Prata:*
First, I think you'd better check your subgroups. Maybe you got opposite
hand of the data.
Or change a model. When the Rfactor is above 0.55 after MR, I think it means
nothing.
On 2/16/07, Emmanuel Prata <[EMAIL PROTECTED]> wrote:
Dear all,
I have a data set of a protein soake
Hey Emmanuel
Typical R-factor for random structure will be above
57% and for initial MR model it will be somewhere
between 40-50%. In your case, High R-factor might be
due to many reasons.
So check the data for any twining and also again check
the Space group. Because sometime you will end up
Dear all,
I have a data set of a protein soaked with sacarose, and the statistic
data is good until the molecular replacement (Rmerge 0,07 (24%),
I/Sigma 7 (2,7), completness (99,8 (98%)), and resolution 1,98A. After
this step, I can not get the Rfactor (64%) to drop (I believe the
spacegroup is
