Dear Jeroen and Shane,
I am quoting from memory only, as I am not as thoroughly familiar
with the crystallisation literature as I would wish to be, but I can
remember David Blow (with whom I started my PhD in 1972) telling me
that, as far as he was aware, the hanging drop vapour diffusion met
A very good source for finding such (and other) information concerning
crystallization of macromolecules is:
Richard Giege, a historical perspective on protein crystallization from
1840 to the present day, FEBS Journal 280 (2013) 6456–6497 !
Vapour diffusion (in this case sitting drop) was fi
Hi ccp4bb,
I've been asked to provide a reference for making hanging-drop crystals,
and I suppose that I never realized the need to cite the technique
specifically. Is there a definitive publication where one could trace the
origins of the technique, or a particularly thorough textbook or review?
