A very good source for finding such (and other) information concerning
crystallization of macromolecules is:
Richard Giege, a historical perspective on protein crystallization from
1840 to the present day, FEBS Journal 280 (2013) 6456–6497 !
Vapour diffusion (in this case sitting drop) was first
mentioned/reported by Hampel A, Labanauskas M, Connors PG, Kirkegard L,
RajBhandary UL, Sigler PB & Bock RM (1968) Single crystals of transfer
RNA from formylmethionine and phenylalanine transfer RNA’s. Science 162,
1384–1387.
Either use the review as a reference or for the hanging drop version of
the vapour technique use:
McPherson, A. (1976) Methods Biochem. Anal. 23, 249-345
or
McPherson A. Crystallization of biological macromolecules. Cold Spring
Harbor, NY: Cold Spring Harbor Laboratory Press; 1999.
(or McPherson A. Preparation and Analysis of Protein Crystals. New York:
Wiley, 1982).
Best
Jeroen
Am 17.03.18 um 07:00 schrieb Shane Caldwell:
Hi ccp4bb,
I've been asked to provide a reference for making hanging-drop
crystals, and I suppose that I never realized the need to cite the
technique specifically. Is there a definitive publication where one
could trace the origins of the technique, or a particularly thorough
textbook or review?
Shane Caldwell
shane.caldwel...@gmail.com <mailto:shane.caldwel...@gmail.com>
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It is invariably the case that high resolution X-ray structures show
significantly better agreement with solution observables such as
coupling constants, 13C chemical shifts, and proton chemical shifts,
than the corresponding NMR structures, including the very best ones.
Hence, in most cases, a high-resolution crystal structure (< 2.0 Å)will
provide a better description of the structure in solution than the
corresponding NMR structure (Kuszewski, Gronenborn & Clore, 1996,
Protein Science 5:1067-80)
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