Re: [ccp4bb] Recovering crystals from dry drops

2014-02-20 Thread Prince, D Bryan
ering your crystals! Regards, Bryan From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Debasish Chattopadhyay Sent: Thursday, February 20, 2014 11:00 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Recovering crystals from dry drops Would you please share your expe

Re: [ccp4bb] Recovering crystals from dry drops

2014-02-20 Thread Miriam Sharpe
Hi Debasish, I had a go at fishing crystals out of dried-out sitting drops some time ago: " The crystals were retrieved from the surrounding viscous liquid by dropping 1 µl cryoprotectant over the drop. This immediately started to thin the liquid, freeing the crystals within, so that one could

Re: [ccp4bb] Recovering crystals from dry drops

2014-02-20 Thread Enrico Stura
other people’s experience really means anything for your crystals. Zhijie From: Debasish Chattopadhyay Sent: Thursday, February 20, 2014 10:59 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Recovering crystals from dry drops Would you please share your experience and comments on recovering

Re: [ccp4bb] Recovering crystals from dry drops

2014-02-20 Thread Zhijie Li
crystals from dry drops Would you please share your experience and comments on recovering protein crystals from dry (or almost dry hanging drops) for data collection. I found some beautiful crystals in hanging drops that were set up three years ago; from the color of the crystals ( the protein

[ccp4bb] Recovering crystals from dry drops

2014-02-20 Thread Debasish Chattopadhyay
Would you please share your experience and comments on recovering protein crystals from dry (or almost dry hanging drops) for data collection. I found some beautiful crystals in hanging drops that were set up three years ago; from the color of the crystals ( the protein binds a colored substrate