Debasish
Assuming you do not need seeds, just lift the coverslip, add a small
amount of water
to the reservoir, close and allow the drop to equilibrate for 20min, or
untill you are sure that you have
enough liquid to avoid that the drops becomes solid while you pick up the
crystals with
a loop and transfer to a suitable cryosolution very rich in cryocomponents
with very little water.
Improved diffraction? 10% chance!
Enrico.
On Thu, 20 Feb 2014 17:21:53 +0100, Zhijie Li <zhijie...@utoronto.ca>
wrote:
Hi Debasish,
I would first use some of those crystals to make seeds and grow some new
crystals so that I would not lose the crystal. Dehydration, even done
systematically (eg,
http://www.mitegen.com/mic_catalog.php?c=jenCrystaloptdehydrate), may or
may not improve the diffraction. Like most other things in biological
crystallography, it varies from case to case. I do not think other
people’s experience really means anything for your crystals.
Zhijie
From: Debasish Chattopadhyay
Sent: Thursday, February 20, 2014 10:59 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Recovering crystals from dry drops
Would you please share your experience and comments on recovering
protein crystals from dry (or almost dry hanging drops) for data
collection.
I found some beautiful crystals in hanging drops that were set up three
years ago; from the color of the crystals ( the protein binds a colored
substrate) and the their shape I know these are crystals of my protein.
I had collected data using some of these crystals when they were fresh;
resolution was poor and the overall I/sigma was low. I am curious if
the dehydration would improve the diffraction now.
Thanks
Debasish
Ph: (205)934-0124; Fax: (205)934-0480
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
