Re: [ccp4bb] Reagent Grade from Hampton

2009-03-26 Thread artem
Hi, Phosphate + calcium - not an easy combination :) Fluka brand PEGs used to be the way to go. I would carefully test the pH of the actual solution in the kit, and compare it with the pH of the solutions you're making yourself. There are several reasons as to why - but these values aren't alway

[ccp4bb] Reagent Grade from Hampton

2009-03-26 Thread Haridasan Namboodiri
Hello, I am trying to reproduce two hits obtained from the PEG/Ion screen of Hampton which showed up with 20% PEG3350 and 0.2M K2HPO4/0.2M CaCl2. I tried optimizations making up my own solutions with the two salts from Sigma (The PEG is from Hampton). To my surprise, the hits do not translate