Re: [ccp4bb] Phosphorylated arginine in COOT

2019-07-27 Thread Paul Emsley
On 27/07/2019 04:47, Anirudha Dutta wrote: From electron density, It looks like one of the arginines in my protein is post-translationally modified as phospho-arginine. How to make sure it's a real phospho-arginine, not an arginine interacting with phosphate or acetate ion? The resolution of

Re: [ccp4bb] Phosphorylated arginine in COOT

2019-07-27 Thread Jonathan Cooper
Hello, can you get a good idea of the distance between the arginine sidechain nitrogen and the phosphorus? It should be less than 2 A for a covalent bond. A non-covalent complex will have an oxygen-nitrogen distance of about 2.8 A. If the Arg is phosphorylated, then this residue is already one o

[ccp4bb] Phosphorylated arginine in COOT

2019-07-26 Thread Anirudha Dutta
Hello everyone >From electron density, It looks like one of the arginines in my protein is post-translationally modified as phospho-arginine. How to make sure it's a real phospho-arginine, not an arginine interacting with phosphate or acetate ion? The resolution of the structure is 2.3 A. Can anybo