On 27/07/2019 04:47, Anirudha Dutta wrote:
From electron density, It looks like one of the arginines in my
protein is post-translationally modified as phospho-arginine. How to
make sure it's a real phospho-arginine, not an arginine interacting
with phosphate or acetate ion? The resolution of the structure is 2.3
A. Can anybody helps me with step by step procedure to incorporate a
phospho-arginine in my structure using COOT...
If you have loaded a dictionary for RPI:
Centre on our ARG.
Entensions -> Modelling -> Replace Residue
RPI
Mutate
If you do not have a dictionary, then you can make one using this mmcif
file and acedrg
http://files.rcsb.org/ligands/download/RPI.cif
Build and refine both hypotheses and compare the residue strain. It
should be a clear answer.
Paul
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